--- abstract: 'Microsurgical repair of transected peripheral nerves is compromised by the formation of scar tissue and the development of a neuroma, thereby limiting the success of regeneration. The aim of this study was to quantify histomorphometrically the structural changes in neural tissue that result from repair, and determine the effect of mannose-6-phosphate (M6P), a scar-reducing agent previously shown to enhance regeneration. In anaesthetised C57-black-6 mice, the left sciatic nerve was sectioned and repaired using four epineurial sutures. Either 100 mu L of 600 mM M6P (five animals) or 100 mu L of phosphate-buffered saline (placebo controls, five animals) was injected into and around the nerve repair site. A further group acted as sham-operated controls. After recovery for 6 weeks, the nerve was harvested for analysis using light and electron microscopy. Analysis revealed that when compared with sham controls, myelinated axons had smaller diameters both proximal and distal to the repair. Myelinated axon counts, axonal density and size all decreased across the repair site. There were normal numbers and densities of non-myelinated axons both proximal and distal to the repair. However, there were more Remak bundles distal to the repair site, and fewer non-myelinated axons per Remak bundle. Application of M6P did not affect any of these parameters.' altloc: [] chapter: ~ commentary: ~ commref: ~ confdates: ~ conference: ~ confloc: ~ contact_email: ~ creators_id: - ngeowy@um.edu.my - ~ - ~ - ~ - ~ - ~ - ~ creators_name: - family: Ngeow given: W.C. honourific: Dr. lineage: '' - family: Atkins given: S. honourific: '' lineage: '' - family: Morgan given: C. R. honourific: '' lineage: '' - family: Metcalfe given: A. D. honourific: '' lineage: '' - family: Boissonade given: F. M. honourific: '' lineage: '' - family: Loescher given: A. R. honourific: '' lineage: '' - family: Robinson given: P. P. honourific: '' lineage: '' date: 2011-11 date_type: published datestamp: 2012-04-25 12:41:26 department: ~ dir: disk0/00/00/81/72 edit_lock_since: ~ edit_lock_until: 0 edit_lock_user: ~ editors_id: [] editors_name: [] eprint_status: archive eprintid: 8172 fileinfo: application/pdf;http://cogprints.org/8172/1/Histomorphometric%20changes%20in%20repaired%20mouse%20sciatic%20nerves%20are%20unaffected%20by%20the%20application%20of%20a%20scar%2Dreducing%20agent.pdf full_text_status: public importid: ~ institution: ~ isbn: ~ ispublished: pub issn: ~ item_issues_comment: [] item_issues_count: ~ item_issues_description: [] item_issues_id: [] item_issues_reported_by: [] item_issues_resolved_by: [] item_issues_status: [] item_issues_timestamp: [] item_issues_type: [] keywords: Histomorphometry; Nerve Regeneration; Nerve Repair; Scarring; Sciatic Nerve; Peripheral-Nerve; Lingual Nerve; Epineurial Sutures; Myelinated Axons; Salivary-Glands; Fibrin Glue; Regeneration; Rat; Size; Cat lastmod: 2012-04-25 12:41:26 latitude: ~ longitude: ~ metadata_visibility: show note: 'Times Cited: 0 Ngeow, Wei Cheong Atkins, Simon Morgan, Claire R. Metcalfe, Anthony D. Boissonade, Fiona M. Loescher, Alison R. Robinson, Peter P.' number: 5 pagerange: 638-645 pubdom: TRUE publication: Journal of Anatomy publisher: 'Wiley-Blackwell, Commerce Place, 350 Main St, Malden 02148, Ma Usa' refereed: TRUE referencetext: ~ relation_type: [] relation_uri: [] reportno: ~ rev_number: 8 series: ~ source: ~ status_changed: 2012-04-25 12:41:26 subjects: - JOURNALS - OJHAS succeeds: ~ suggestions: "Associate Prof. Dr. Ngeow Wei Cheong @ David Ngeow\r\nDepartment of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of\r\nMalaya, 50603 Kuala Lumpur, MALAYSIA\r\n\r\nAssociate Professor Dr. Ngeow Wei Cheong is currently a lecturer at the Department of Oral and Maxillofacial Surgery, Faculty of Dentistry of the University of Malaya. He graduated from the University of Malaya in 1992 and was a private practitioner for 9 months before he was offered the post of tutorship at his alma matter. Three years later, he went to do a house job at the Queen Victoria Hospital in East Grinstead, England. He obtained his Fellowship in Dental Surgery from the Royal College of Surgeons in Ireland and the Royal College of Surgeons ofEngland in 1996. He subsequently returned to Malaysia and was a pioneer lecturer at the then newly established Universiti Kebangsaan Malaysia. He left for private practice in 1999 but the temptation to return to academic life was greater, hence he returned to the University of Malaya the subsequent year. His research interest are craniofacial anthropometry, variations of the mandibular nerve, and recovery of peripheral nerves after microsurgical repair. \r\n \r\n\r\nAREAS OF EXPERTISE\r\n\t\r\nDentofacial Anthropometry (Head & Neck, anthropology)\r\n\r\nDental Analgesics (analgesic, local anaesthetic, COX-2 inhibitors)\r\n\r\nSurgical Anatomy (mental foramen, anterior loop)" sword_depositor: ~ sword_slug: ~ thesistype: ~ title: Histomorphometric changes in repaired mouse sciatic nerves are unaffected by the application of a scar-reducing agent type: journalp userid: 16514 volume: 219