?url_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rft.title=Histomorphometric+changes+in+repaired+mouse+sciatic+nerves+are+unaffected+by+the+application+of+a+scar-reducing+agent&rft.creator=Ngeow%2C+Dr.+W.C.&rft.creator=Atkins%2C+S.&rft.creator=Morgan%2C+C.+R.&rft.creator=Metcalfe%2C+A.+D.&rft.creator=Boissonade%2C+F.+M.&rft.creator=Loescher%2C+A.+R.&rft.creator=Robinson%2C+P.+P.&rft.subject=JOURNALS&rft.subject=Online+Journal+of+Health+and+Allied+Sciences&rft.description=Microsurgical+repair+of+transected+peripheral+nerves+is+compromised+by+the+formation+of+scar+tissue+and+the+development+of+a+neuroma%2C+thereby+limiting+the+success+of+regeneration.+The+aim+of+this+study+was+to+quantify+histomorphometrically+the+structural+changes+in+neural+tissue+that+result+from+repair%2C+and+determine+the+effect+of+mannose-6-phosphate+(M6P)%2C+a+scar-reducing+agent+previously+shown+to+enhance+regeneration.+In+anaesthetised+C57-black-6+mice%2C+the+left+sciatic+nerve+was+sectioned+and+repaired+using+four+epineurial+sutures.+Either+100+mu+L+of+600+mM+M6P+(five+animals)+or+100+mu+L+of+phosphate-buffered+saline+(placebo+controls%2C+five+animals)+was+injected+into+and+around+the+nerve+repair+site.+A+further+group+acted+as+sham-operated+controls.+After+recovery+for+6+weeks%2C+the+nerve+was+harvested+for+analysis+using+light+and+electron+microscopy.+Analysis+revealed+that+when+compared+with+sham+controls%2C+myelinated+axons+had+smaller+diameters+both+proximal+and+distal+to+the+repair.+Myelinated+axon+counts%2C+axonal+density+and+size+all+decreased+across+the+repair+site.+There+were+normal+numbers+and+densities+of+non-myelinated+axons+both+proximal+and+distal+to+the+repair.+However%2C+there+were+more+Remak+bundles+distal+to+the+repair+site%2C+and+fewer+non-myelinated+axons+per+Remak+bundle.+Application+of+M6P+did+not+affect+any+of+these+parameters.&rft.publisher=Wiley-Blackwell%2C+Commerce+Place%2C+350+Main+St%2C+Malden+02148%2C+Ma+Usa&rft.date=2011-11&rft.type=Journal+(Paginated)&rft.type=PeerReviewed&rft.format=application%2Fpdf&rft.identifier=http%3A%2F%2Fcogprints.org%2F8172%2F1%2FHistomorphometric%2520changes%2520in%2520repaired%2520mouse%2520sciatic%2520nerves%2520are%2520unaffected%2520by%2520the%2520application%2520of%2520a%2520scar-reducing%2520agent.pdf&rft.identifier=++Ngeow%2C+Dr.+W.C.+and+Atkins%2C+S.+and+Morgan%2C+C.+R.+and+Metcalfe%2C+A.+D.+and+Boissonade%2C+F.+M.+and+Loescher%2C+A.+R.+and+Robinson%2C+P.+P.++(2011)+Histomorphometric+changes+in+repaired+mouse+sciatic+nerves+are+unaffected+by+the+application+of+a+scar-reducing+agent.++%5BJournal+(Paginated)%5D+++++&rft.relation=http%3A%2F%2Fcogprints.org%2F8172%2F