--- abstract: 'We have investigated the effect of three potential scar-reducing agents applied at a sciatic nerve repair site in C57-black-6 mice. Under anaesthesia the nerve was transected, repaired using four epineurial sutures, and 100 mu l of either triamcinolone acetonide (1 mg/100 mu l), an interleukin-10 peptide fragment: (125 ng/100 mu l or 500 ng/100 mu l) or mannose-6-phosphate (M6P, 200 mM or 600 mM) was injected into and around the nerve. After 6 weeks the extent of regeneration was assessed electrophysiologically by determining the ratio of the compound action potential (CAP) modulus evoked by electrical stimulation of the nerve 2 mm distal or proximal to the repair site. The conduction velocity of the fastest components in the CAP was also calculated. The percentage area of collagen staining (PAS) at the repair site was analysed using Picrosirius Red and image analysis. Comparisons were made with a placebo group (100 mu l of phosphate buffered saline) and sham-operated controls. The median CAP modulus ratio in the 600 mM M6P group was 0.44, which was significantly higher than in the placebo group (0.24, P=0.012: Kruskal-Wallis test). Conduction velocities were also faster in the 600 mM M6P group (median 30 m s(-1)) than in the placebo group (median 27.8 m s(-1); P=0.0197: Kruskal-Wallis test). None of the other treated groups were significantly different from the placebo, and all had significantly lower CAP ratios than the sham controls (P{\ensuremath{<}}0.05). All repair groups had a significantly higher PAS for collagen than sham controls. We conclude that the administration of 600 mM mannose-6-phosphate to a nerve repair site enhances; axonal regeneration. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.' altloc: [] chapter: ~ commentary: ~ commref: ~ confdates: ~ conference: ~ confloc: ~ contact_email: ~ creators_id: - ngeowy@um.edu.my - ~ - ~ - ~ - ~ - ~ - ~ creators_name: - family: Ngeow given: W.C. honourific: Dr. lineage: '' - family: Atkins given: S. honourific: '' lineage: '' - family: Morgan given: C. R. honourific: '' lineage: '' - family: Metcalfe given: A. D. honourific: '' lineage: '' - family: Boissonade given: F. M. honourific: '' lineage: '' - family: Loescher given: A. R. honourific: '' lineage: '' - family: Robinson given: P. P. honourific: '' lineage: '' date: 2011-05 date_type: published datestamp: 2012-04-25 13:03:30 department: ~ dir: disk0/00/00/81/69 edit_lock_since: ~ edit_lock_until: 0 edit_lock_user: ~ editors_id: [] editors_name: [] eprint_status: archive eprintid: 8169 fileinfo: application/pdf;http://cogprints.org/8169/1/A%20COMPARISON%20BETWEEN%20THE%20EFFECTS%20OF%20THREE%20POTENTIAL%20SCAR.pdf full_text_status: public importid: ~ institution: ~ isbn: ~ ispublished: pub issn: ~ item_issues_comment: [] item_issues_count: ~ item_issues_description: [] item_issues_id: [] item_issues_reported_by: [] item_issues_resolved_by: [] item_issues_status: [] item_issues_timestamp: [] item_issues_type: [] keywords: 'Peripheral Nerve Repair; Nerve Regeneration; Scarring; Triamcinolone Acetonide; Interleukin-10; Mannose-6-Phosphate; Growth-Factor-Beta; Peripheral-Nerve; Triamcinolone Acetonide; Intralesional Injection; Messenger-Rna; Schwann-Cells; Regeneration; Collagen; Injury; Interleukin-10 ' lastmod: 2012-04-25 13:03:30 latitude: ~ longitude: ~ metadata_visibility: show note: 'Times Cited: 3 Ngeow, W. C. Atkins, S. Morgan, C. R. Metcalfe, A. D. Boissonade, F. M. Loescher, A. R. Robinson, P. P.' number: ~ pagerange: 271-277 pubdom: TRUE publication: Neuroscience publisher: 'Publisher: PERGAMON-ELSEVIER SCIENCE LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND' refereed: TRUE referencetext: ~ relation_type: [] relation_uri: [] reportno: ~ rev_number: 8 series: ~ source: ~ status_changed: 2012-04-25 13:03:30 subjects: - JOURNALS - OJHAS succeeds: ~ suggestions: "Associate Prof. Dr. Ngeow Wei Cheong @ David Ngeow\r\nDepartment of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of\r\nMalaya, 50603 Kuala Lumpur, MALAYSIA\r\n\r\nAssociate Professor Dr. Ngeow Wei Cheong is currently a lecturer at the Department of Oral and Maxillofacial Surgery, Faculty of Dentistry of the University of Malaya. He graduated from the University of Malaya in 1992 and was a private practitioner for 9 months before he was offered the post of tutorship at his alma matter. Three years later, he went to do a house job at the Queen Victoria Hospital in East Grinstead, England. He obtained his Fellowship in Dental Surgery from the Royal College of Surgeons in Ireland and the Royal College of Surgeons ofEngland in 1996. He subsequently returned to Malaysia and was a pioneer lecturer at the then newly established Universiti Kebangsaan Malaysia. He left for private practice in 1999 but the temptation to return to academic life was greater, hence he returned to the University of Malaya the subsequent year. His research interest are craniofacial anthropometry, variations of the mandibular nerve, and recovery of peripheral nerves after microsurgical repair. \r\n \r\n\r\nAREAS OF EXPERTISE\r\n\t\r\nDentofacial Anthropometry (Head & Neck, anthropology)\r\n\r\nDental Analgesics (analgesic, local anaesthetic, COX-2 inhibitors)\r\n\r\nSurgical Anatomy (mental foramen, anterior loop)" sword_depositor: ~ sword_slug: ~ thesistype: ~ title: A comparison between the effects of three potential scar-reducing agents applied at a site of sciatic nerve repair type: journalp userid: 16514 volume: 181