creators_name: Patil, P creators_name: Gaikwad, RD creators_name: Sawane, MV creators_name: Waghmare, VS creators_id: prashantpatil1411@gmail.com editors_name: Kakkilaya, Srinivas editors_id: Kakkilaya BS type: journale datestamp: 2010-09-13 03:53:55 lastmod: 2011-03-11 08:57:42 metadata_visibility: show title: Effect of Neem Oil on Sperm Mitochondrial Activity ispublished: pub subjects: OJHAS full_text_status: public keywords: Normozoospermia, Neem Oil, Sperm Mitochondrial Activity abstract: It is a known fact that neem oil has some effect on motility of sperm. Motility of sperm depends on mitochondrial activity present in mid-piece of sperm. In the present study, the mitochondrial activity of sperm was evaluated after treating semen with the different quantities of neem oil. The mitochondrial activity was also evaluated after subjecting the semen samples for different incubation periods keeping the quantity of semen as well as that of neem oil same. Tests were done on thirty normozoospermic semen samples with motile score more than 75%. It was found that as the quantity of neem oil increases, the mitochondrial activity decreases significantly (P < 0.001). Similar results were found, when same quantity of neem oil was treated with same quantity of semen, but incubating for different time durations. The mitochondrial activity decreases significantly (P < 0.001) from one minute to twenty minutes. So, it indicates that as the contact period between neem oil and semen increases the mitochondrial activity decreases significantly date: 2010-04-30 date_type: published publication: Online Journal of Health and Allied Sciences volume: 8 number: 4 publisher: Dr. BS Kakkilaya refereed: TRUE referencetext: 1. Sinha KC et al. Anti-implantation effect of neem oil. Ind. J M. Res. 1984;80;708-710. 2. Sharma VN, Saxena KP. Spermicidal action of Sodium Nimbinate. Ind Jour. M. Res 1959;47;322-324. 3. Sinha KC, Tiwari RS. Neem oil as Vaginal Contraceptive. Ind Jour. M. Res Jan1984;79;131-136. 4. Gopalkrishna et al. Mol. Andrology 1990;3;243-253. 5. Sharma VN et al. Ind Jour. M. Res 1959;47; 3. 6. Kasturi M, Ahmad RN et al. Mode of long term anti-fertility effect of intra-uterine neem treatment. J Basic Clin Physiol Pharmacol. 2002,13(4):311:28. 7. Sharma SK, Sairam M et al. Neem oil inhibits two cell embryo development and trophectoderm attachment and proliferation in vitro. Contraception. Dec 1996;54(6):373–8. 8. Juneja SC, Williams RS et al. Immunocontraception undergoing promising trials. J Assist Reprod Genet. Aug 1996;13(7):576–85. 9. Mitra CR. In-Natural Pesticides from neem tree, proceedings of 1st International conference, Rottach Egren, Federal Republic of Germany, 16-18 June 1980. pg no. 97. 10. Rembold H, Seiber KP. In-Natural Pesticides from neem tree, proceedings of 1st International conference, Rottach Egren, Federal Republic of Germany, 16-18 June 1980. pg no. 75. 11. Chopra et al. Indigenous Drugs of India, 1982 Pg no. 962. 12. Newton P et al. A new family planning tool to slow population growth. IDRC Rep. Jan 1993;20(4):16–18. 13. Bardhan J, Riar SS et al. Neem oil – a fertility controlling agent in rhesus monkey. IJPP. Oct 1991;35(4):278–80. citation: Patil, P and Gaikwad, RD and Sawane, MV and Waghmare, VS (2010) Effect of Neem Oil on Sperm Mitochondrial Activity. [Journal (On-line/Unpaginated)] document_url: http://cogprints.org/6984/1/2009-4-12.pdf