creators_name: Mungli, Prakash creators_name: Phani, Nagaraj M creators_name: R, Kavya creators_name: M, Supriya creators_id: prakashmungli@yahoo.co.in editors_name: Kakkilaya, Srinivas editors_id: Kakkilaya BS type: journale datestamp: 2010-11-22 14:20:09 lastmod: 2011-03-11 08:57:41 metadata_visibility: show title: Urinary Peptide Levels in Patients with Chronic Renal Failure ispublished: pub subjects: OJHAS full_text_status: public keywords: Peptiduria; Proteinuria; Chronic renal failure; Urinary peptides abstract: Introduction: Peptide levels in urine are found to be decreased in renal failure. In the current study urinary peptide levels were determined in chronic renal failure (CRF) patients. Method: 86 CRF patients and 80 healthy controls were selected for the study. Urinary proteins and peptide levels were determined by spectrophotometer based Lowry and Bradford methods. Urinary creatinine levels were determined by clinical chemistry analyzer. Results: There was significant decrease in urinary peptide levels in CRF patients and Urinary % peptides were significantly decreased in CRF patients as compared to healthy controls. Urinary % peptides correlated negatively with proteinuria. Conclusion: we have found decrease in urinary peptides and % urinary peptides in CRF patients and possibly measurement of % urinary peptides may possibly serve as better indicator in early detection of impairment in renal function. date: 2010-10-15 date_type: published publication: Online Journal of Health and Allied Sciences volume: 9 number: 3 publisher: Dr. B.S. Kakkilaya refereed: TRUE referencetext: 1. Skorecki K, Green J, Brenner BM. Chronic renal failure. In: Harrisons principles of internal medicine, Kasper DL, Braunwald E, Fauci AS (Editors), 16th edition, McGraw-Hill, New York, page 1663-7. 2. Gerstein HC, Mann JF, Yi Q. Albuminuria and risk of cardiovascular events, death and heart failure in diabetic and nondiabetic individuals. JAMA 2001;286:421-6. 3. Mann JF, Gerstein HC, Pogue J, Bosch J, Yusuf S. Renal insufficiency as a predictor of cardiovascular outcomes and the impact of ramipril: the HOPE randomized trial. Ann Intern Med 2001;134:629-36. 4. Burton C, Harris KPG. The role of proteinuria in the progression of chronic renal failure. Am J Kidney Dis 1996;27:765-75. 5. Lemley K. Proteinuria or peptiduria: where’s the beef? J Lab Clin Med 2005;145:228-230. 6. Maack T, Park CH, Camargo MJ. Renal filtration and metabolism of proteins. In: The Kidney, physiology and pathophysiology. Seldin DW, Giebish G (editors), New York, 1985: page 1773-804. 7. Gudehithlu KP, Pegoraro AA, Dunea G, Arruda JAL, Singh A. Degradation of albumin by the renal proximal tubule cell and the subsequent fate of its fragments. Kidney Int 2004;65:2113-2122. 8. Osicka TM, Pratt LM, Comper WD. Glomerular capillary wall permeability to albumin and horseradish peroxidase. Nephrology 1996;2:199-212. 9. Osicka TM, Comper WD. Protein degradation during renal passage in normal kidneys is inhibited in experimental albuminuria. Clin Sci 1997;93:65-72. 10. Osicka TM, Panagiotopoulos S, Jerums G, Comper WD. Fractional clearance of albumin is influenced by albumin degradation. Clin Sci 1997;93:557-564. 11. Burne MJ, Panagiotopoulos S, Jerums G, Comper WD. Alterations in renal Degradation of albumin in early experimental diabetes: a new factor in the mechanism of albuminuria. Clin Sci 1998;95:67-72. 12. Prakash M, Shetty JK, Dash S, Barik BK, Sarkar A, Prabhu R. Determination of urinary peptides in patients with proteinuria. Indian J Nephrol 2008;18:150-54. 13. Lowry OH, Rosenbrough NJ, Farr AI, Randall RT. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-75. 14. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of protein dye binding. Anal Biochem 1976;72:248-54. 15. Russo LM, Bakris G, Comper W. Renal handling of albumin: a critical review of basic concept and perspective. Am J Kidney Dis 2002;39:899-919. 16. Singh A, Gudehithlu KP, Le G, Litbarg NO, Khalili V, Vernik J, Hart P, Arruda JAL, Dunea G. Decreased urinary peptide excretion in patients with renal disease. Am J Kidney Dis. 2004;44:1031-1038. citation: Mungli, Prakash and Phani, Nagaraj M and R, Kavya and M, Supriya (2010) Urinary Peptide Levels in Patients with Chronic Renal Failure. [Journal (On-line/Unpaginated)] document_url: http://cogprints.org/6969/1/2010-3-5.pdf