<> "The repository administrator has not yet configured an RDF license."^^ . <> . . "Post-ischaemic treatment with the cyclooxygenase-2 inhibitor nimesulide reduces blood-brain barrier disruption and leukocyte infiltration following transient focal cerebral ischaemia in rats"^^ . "Several studies suggest that cyclooxygenase (COX)-2 plays a pivotal role in the progression of ischemic brain damage. In the present study, we investigated the effects of selective inhibition of COX-2 with nimesulide (12 mg/kg) and selective inhibition of COX-1 with valeryl salicylate (VAS, 12-120 mg/kg) on prostaglandin E2 (PGE2) levels, myeloperoxidase (MPO) activity, Evans Blue (EB) extravasation and infarct volume in a standardized model of transient focal cerebral ischemia in the rat. Postischemic treatment with nimesulide markedly reduced the increase in PGE2 levels in the ischemic cerebral cortex 24 h after stroke and diminished infarct size by 48 % with respect to vehicle-treated animals after 3 days of reperfusion. Furthermore, nimesulide significantly attenuated the blood-brain barrier (BBB) damage and leukocyte infiltration (as measured by EB leakage and MPO activity, respectively) seen at 48 h after the initial ischemic episode. These studies provide the first experimental evidence that COX-2 inhibition with nimesulide is able to limit BBB disruption and leukocyte infiltration following transient focal cerebral ischemia. Neuroprotection afforded by nimesulide is observed even when the treatment is delayed until 6 h after the onset of ischemia, confirming a wide therapeutic window of COX-2 inhibitors in experimental stroke. On the other hand, selective inhibition of COX-1 with VAS had no significant effect on the evaluated parameters. These data suggest that COX-2 activity, but not COX-1 activity, contributes to the progression of focal ischemic brain injury, and that the beneficial effects observed with non-selective COX inhibitors are probably associated to COX-2 rather than to COX-1 inhibition. "^^ . "2007-02" . . "100" . "4" . . "Blackwell Publishing"^^ . . . "Journal of Neurochemistry"^^ . . . . . . . . . . . . . . . . . . . . . . . . . . "Sean"^^ . "Murphy"^^ . "Sean Murphy"^^ . . "Olga Sonia"^^ . "Leon"^^ . "Olga Sonia Leon"^^ . . "Eduardo"^^ . "Candelario-Jalil"^^ . "Eduardo Candelario-Jalil"^^ . . "Bernd"^^ . "Fiebich"^^ . "Bernd Fiebich"^^ . . "Anthony"^^ . "Turner"^^ . "Anthony Turner"^^ . . "Michel"^^ . "Garcia-Cabrera"^^ . "Michel Garcia-Cabrera"^^ . . "Armando"^^ . "Gonzalez-Falcon"^^ . "Armando Gonzalez-Falcon"^^ . . . . . . "Post-ischaemic treatment with the cyclooxygenase-2 inhibitor nimesulide reduces blood-brain barrier disruption and leukocyte infiltration following transient focal cerebral ischaemia in rats (PDF)"^^ . . . . . . . . . "J_Neurochem_2007_Candelario-Jalil_et_al.pdf"^^ . . . "Post-ischaemic treatment with the cyclooxygenase-2 inhibitor nimesulide reduces blood-brain barrier disruption and leukocyte infiltration following transient focal cerebral ischaemia in rats (Image (PNG))"^^ . . . . . . "preview.png"^^ . . . "Post-ischaemic treatment with the cyclooxygenase-2 inhibitor nimesulide reduces blood-brain barrier disruption and leukocyte infiltration following transient focal cerebral ischaemia in rats (Indexer Terms)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #5393 \n\nPost-ischaemic treatment with the cyclooxygenase-2 inhibitor nimesulide reduces blood-brain barrier disruption and leukocyte infiltration following transient focal cerebral ischaemia in rats\n\n" . "text/html" . . . "Neurochemistry" . .