%A Enabulele I O
%A Yah S C
%A Yusuf E O
%A Eghafona N O
%J Online Journal Of Health And Allied Sciences
%T Emerging quinolones resistant transfer genes among gram-negative bacteria, isolated from faeces of HIV/AIDS patients attending some Clinics and Hospitals in the City of Benin, Edo State, Nigeria
%X A survey of 1431 gram-negative bacilli from June 2001 to September 2005 were obtained from the faeces of 920 HIV/AIDS patients attending some Clinics and Hospitals in Benin City, Nigeria, were screened for quinolones resistance gene. The HIV/AIDS patients CD4 cells range was ?14/mm3 ?800/mm3 of blood. Out of the 1431 isolates, 343 (23.9%) were resistance to quinolones with a MIC ?4?g/ml for norfloxacin, ciprofloxacin and pefloxacin while a MIC of ?32 ?g/ml for nalidixic acid. The screened isolates include Pseudomonas aeruginosa 64(18.7%), E coli 92(26.8%), Klebsiella pneumoniae 53(15.4%), Salmonella typhi 39(11.4%), Shigella dysenteriae 36(10.5%), Proteus mirabilis 34(9.9%) and Serratia marcescens 25(7.3%).  The average resistance of the isolates to the various quinolones ranged from 42.7% to 66.7%.  Klebsiella were the most resistant isolates with a mean resistance of 66.7% while Proteus were the less resistant isolates with a mean resistance of 42.7%. Most isolates were resistant to Nalidixic acid followed by norfloxacin while the less resistant were to the pefloxacin. The frequency of qnr genes transfer to EJRifr as recipient ranged from 2 x 10-2 to 6 x 10-6 with an average of 2 plasmids per cell. The molecular weight of the plasmids ranged from <2.9kbp to <5.5 kbp. This indicated that plasmids allowed the movement of genetic materials including qnr resistant genes between bacteria species and genera in Benin City, Nigeria
%N 3
%K Resistance Gene Transfer, HIV/AIDS, Gram-negative bacilli
%E Dr. Srinivas Kakkilaya Bevinje
%V 5
%D 2006
%I Dr. B.S. Kakkilaya
%L cogprints5322

<script src="//archive-bar.soton.ac.uk/archive-bar.js"></script>