Cogprints

AZF Microdeletions in Human Semen Infected with Bacteria

Hassani, HH and Khalaf, ZSH and Samarie, EJ and Ibrahim, MA. (2011) AZF Microdeletions in Human Semen Infected with Bacteria. [Journal (On-line/Unpaginated)]

Full text available as:

[img]
Preview
 PDF - Published Version
Available under License Creative Commons Attribution No Derivatives.
148Kb

Abstract

Bacterial infections are associated with infertility in men. This study was aimed to investigate microdeletions on Yq chromosome in semen infected with bacteria by using bacteriological, biochemical, and serological assays. The investigation showed that 107 of 300 (84.80%) semen samples collected from infertile men with primary or secondary infertility were infected with different species of bacteria. Chlamydia trachomatis and Neisseria gonorrheae were the most frequently diagnosed bacteria in the infected semen samples. The percentages of infections of semen samples with C. trachomatis and N. gonorrhea were 42.31% and 35.28% respectively.  Genomic DNA from each semen sample infected with predominant bacteria was analyzed for AZF deletions by using multiplex PCR. Different patterns of AZF microdeletions were obtained. It can be concluded that sexually transmitted bacteria may contribute in microdeletions of Yq chromosome by indirectly producing reactive oxygen species and causing gene defect in AZF regions.

Item Type:Journal (On-line/Unpaginated)
Keywords:Male infertility; Yq microdeletion, Chlamydia trachomatis, Neisseria gonorrheae
Subjects:JOURNALS > Online Journal of Health and Allied Sciences
ID Code:8005
Deposited By: Kakkilaya Bevinje, Dr. Srinivas
Deposited On:09 Nov 2012 17:37
Last Modified:09 Nov 2012 17:37

References in Article

Select the SEEK icon to attempt to find the referenced article. If it does not appear to be in cogprints you will be forwarded to the paracite service. Poorly formated references will probably not work.

1. Dohle GR, Halley DJ, Van Hemel JO et al. Genetic risk factors in infertile men with sever oligozoospermia and azoospermia. Hum Reprod. 2002;17:13-16

2. Golshani M, Taheri S, Eslami G et al. Genital tract infection in asymptomatic infertile men and its effect on semen quality. Iranian J Publ Health. 2006;35(3):81-84

3. Tiepolo L, Zuffardi O. Localization of factors control lingospermatogenesis in the nonfluorescent portion of the human Y chromosome long arm. Hum Gene. 1976;34:119-124

4. Vogt PH, Edelmann A, Kirsch S et al. Human Y chromosome azoospermia factors (AZF) mapped to different subregions in Yq11. Hum Mol Genet. 1996;5:933-943

5. Kin CM. A study on the prevalence of AZFd Y-chromosome microdeletion in Hong Kong Chinese men with severe male factor infertility. Med Sc Dissertation submitted to the University of Hong Kong. 2004;21-22

6. Kilic S, Yuksel B, Yilmaz N et al. Results of ICSI in severe oligozoospermic and azoospermic patients with AZF microdeletions. Iranian J Reprod Med. 2009;7 (2):79-84

7. Ferlin A, Arredi B, Speltra E et al. Molecular and clinical characterization of Y chromosome microdeletions in infertile men: A 10-year experience in Italy. J Clin Endocrinol Metab. 2007;92(3):762-770

8. Ibadin OK and Ibeh IN. Bacteriospermia and sperm quality in infertile male patient at University of Benin Teaching Hospital, Benin City, Nigeria. Malaysian Jl. 2008;4(2):65-67

9. Marconi M, Pilatz A, Wagenlehner et al. Impact of infection on the secretory capacity of the male accessory glands. Int Braz J Urol. 2009;35(3):299-309

10. Onemu SO, Ogbimi AO, Ophori EA. Microbiology and semen incidices of sexually-active males in Benin City, Edo state, Nigeria. J Bacteriol Res. 2010;2(5):55-59

11. World Health Organization (WHO). Basic laboratory procedures in clinical bacteriology, 2nd ed. Geneva

12. Holt J, Krieg N, Sneath P et al. Bergey’s Manual of Determinative Bacteriology, 9th ed. Baltimore: Williams & Wilkins; 1994.

13. Collee J, Marmion B, Fraser A et al. Practical medical microbiology. 4th ed. Churchill illivingstone; 1996.

14. Prescott LM, Harley JP and Klein DA. Microbiology. 6th ed. McGraw-Hill Higher Education Press; 2005.

15. Winn WC, Koneman EW. Konemans color atlas and text book of diagnostic microbiology. 6th ed. Lippincott Williams & Wilkins; 2006.

16. Kraube B, Dollmann P, Zeidler H, Kuiper J. Frequent contamination of C. trachomatis and C. pneumoniae strains with Mycoplasma. Biological relevance and selective eradication of Mycoplasma from Chlamydia cultures. Med Microbiol Immunol. 2000;189:19-26

17. Simoni M, Bakker E, Eurlings et al. Laboratory guidelines for molecular diagnosis of Y-chromosomal microdeletions. Int J Androl. 1999;22:292-299

18. Wang RX, Fu C, Yang YP et al. Male infertility in China: Laboratory finding for AZF microdeletions and chromosomal abnormalities in infertile men from Northeastern China. J Assist Reprod Genet. 2010;27:391-396

19. Askienazy-Elnhar. Male genital tract infection: the point of view of the bacteriologist. Gynecol Obstetrique and Fertilite. 2005;33(9):691-697

20. Okonofua FE. Female and male infertility in Nigeria. From Dept Public Health Sciences, Karolinska Institutet, Stockholm, Sweden; 2005. PP.68

21. Hosseinzadeh S, Pacey AA, Eley A. Chlamydia trachomatis-induced death of human spermatozoa is caused primarily by liposaccharide. J Med Microbiol. 2003;52:193-200

22. Gomez CI, Stenback WA, James AN et al. Attachment of Neisseria gonorrheae to human sperm. Microscopical study of tysin and iron. Br J Vener Dis. 1979;55:245-255

23. Kauer S, Prabba V, Shukla G, Sarwal A.  Interference of human spermatozoa motility by live Staphylococcus aureus. Am J Biomed Sci. 2010;2(1):91-97

24. Adejuwon AO, Bisi-Johnson MA, Ajayi AO et al. Lipid levels in men infected with Treponema pallidum. Insight Microbiol. 2011;1(2):31-33

25. Ochsendorf FR. Infections in the male genital tract and reactive oxygen species. Hum Reprod Update. 1999;5(5):399-420

26. Shamsi MB, Venkatesh S, Pathak D et al. Sperm DNA damage and oxidative stress in recurrent spontaneous abortion (RSA). Ind J Med Res. 2011;133:550-551

27. Zalata AA, Ahmed AH, Allamaneni SS et al. Relationship between acrosin activity of human. Asian J Androl. 2004;6:313-318

28. Trum JW, Mol BW, Pannekock Y et al. Value of detecting spermatozoa and oxidative stress leukocytospermia in the diagnosis of genital tract infection in subfertile men. Fertil Steril. 1998;70:315-339

29. Agarwal A, Prabakaran SA, Said TM. Prevention of oxidative stress injury to sperm. J Androl. 2005;26(6):654-660.

Metadata

Repository Staff Only: item control page

Cogprints is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.