Cogprints

Modulation of DMBA- induced biochemical and histopathological changes by Syzygium cumini seed extract during skin carcinogenesis

Jyoti Parmar , Priyanka Sharma , Preeti Verma , Priyanka Sharma , P.K.Goyal , Int J Cur Biomed Phar Res. (2011) Modulation of DMBA- induced biochemical and histopathological changes by Syzygium cumini seed extract during skin carcinogenesis. [Journal (Paginated)]

Full text available as:

[img]
Preview
PDF (Modulation of DMBA- induced biochemical and histopathological changes by Syzygium cumini seed extract during skin carcinogenesis) - Published Version
Available under License Creative Commons Attribution No Derivatives.

1505Kb

Abstract

Aim & Method: The current study was designed to elucidate the protective effect of Syzygium cumini seed extract (SCE) on skin carcinogenesis induced by a single topical application of 7,12-dimethylbenz(a)anthracene (100 μg/100 μl of acetone) and 2 weeks later promoted by repeated application of croton oil (1% in acetone/three times a week) till the end of the experiment (16 weeks). Result: Oral administration of SCE at a dose of 125 mg/kg b.wt./day for 15 days at the peri-initiational stage (i.e., 7 days before & 7 days after DMBA application) and for 14 weeks at the promotional stage (i.e., from the time of croton oil application), revealed a significant reduction in lipid peroxidation (p<0.05-0.001) along with an elevation in the activities of enzymatic antioxidants (superoxide dismutase, p<0.05-0.001 & catalase, p<0.05-0.001), non-enzymatic antioxidant (reduced glutathione, p<0.05-0.01 & vitamin-C, p<0.01-0.001) and total proteins levels (p<0.01-0.001) when compared to the carcinogen treated control animals. Histopathological study revealed that dyskeratosis of the epidermis, deposition of keratinous pearl and epidermal hyperplasia in skin tumors of DMBA treated control and the same were found to be of lesser degree in both the SCE treated experimental animals. Conclusions: These results demonstrate that SCE ameliorate the DMBA/croton oil induced adverse biochemical and histopathological alterations during skin carcinogenesis in mice.

Item Type:Journal (Paginated)
Keywords:Antioxidant enzymes, carcinogenesis, Hyperplasia, Keratinous Pearl, Lipid per-oxidation, Skin tumor, Syzygium cumini.
Subjects:JOURNALS
ID Code:7328
Deposited By:CurrentSciDirect Publications, International Journal of Current Biomedical and Pharmaceutical Research (IJCBPR)
Deposited On:02 May 2011 16:51
Last Modified:02 May 2011 16:51

References in Article

Select the SEEK icon to attempt to find the referenced article. If it does not appear to be in cogprints you will be forwarded to the paracite service. Poorly formated references will probably not work.

[1] De Flora S, Lzzotti A, Agostini FD, Balansky RM, Noonan D, Albini A. Multiple points of intervention in the prevention of cancer and other mutation related diseases. Mutat. Res. 2001; 9: 480–481.

[2] Dipple A, Moschel RC, Bigger CAH. Polynuclear aromatic carcinogens. American Chemical Society Monograph 182, American Chemical Society, Washington, DC, Chemical Carcinogens (C. E. Searle, eds), 1984; 1: 41-163.

[3] Yang SK, Silverman BD. (eds). Polycyclic Aromatic Hydrocarbon Carcinogenesis: Structure-Activity Relationships, CRC Press, Boca Raton, FL. 1988: 1: 2.

[4] Harvey RG. Polycyclic Aromatic Hydrocarbons, Wiley-VCH, New York, 1997.

[5] Conney AH. Induction of Microsomal Enzymes by Foreign Chemicals and Carcinogenesis by Polycyclic Aromatic Hydrocarbons. Cancer Res. 1982; 42: 4875-4917.

[6] Slattery ML, O'Brien E, Mori M. Disease heterogeneity: does it impact our ability to detect dietary associations with breast cancer? Nutr Cancer. 1995; 24: 213-220.

[7] Ray G, Batra S, Shukla NK, Deo S, Raina V, Ashok S and Hu Sain SA. Lipid peroxidation, free radical production and antioxidant status in breast cancer, Breast Cancer Res., Treat. 2000; 59: 163-170.

[8] Halliwell B, Gutteridge JMC. Free Radicals in Biology and Medicine. Oxford, UK: Clarendon. 1989; 96-98.

[9] The Ayurvedic Pharmacopoeia of India, Part-I, 1st edition, Vol-II, The controller of publications, Delhi. 1999; 54-57.

[10] Brahmachari HD, and Augusti KT. Hypoglycemic agents from Indian indigenous plants. Journal of Pharmacy and Pharmacology. 1961; XIII (1): 143.

[11] Kaur Ajit, Choudhary BK and Bandyopadhyay NG. Comparative evaluation of hypoglycaemic activity of some Indian medicinal plants in alloxon diabetic rats. Journal of ethnopharmacology. 2003; 84: 105-108.

[12] Pepato MT, Mori DM, Baviera AM, Harami JB, Vendramini RC and Brunetti LL. Fruit of the jambolan tree (Eugenia jambolana Lam.) and experimental diabetes. Journal of Ethanopharmacol. 2005; 96: 43-48.

[13] Slaga TG, Budunova IV, Gimenez, Conti IB, Aldaz CM. The mouse skin chemical carcinogenesis model. J Investig Dermatol Symp Proc. 1996; 1: 151-156.

[14] Ohkhawa H, Ohishi N and Yogi K. Assay for lipid peroxidation in animal tissue by thiobarbituric acid reaction. Analyt. Biochem. 1979; 95: 351-358.

[15] Moron MA, Depierre JW, Mannervick B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochem Biophys Acta. 1979; 582:67-78.

[16] Aebi H. Catalase: in vitro. In: S.P.Colowick, N.O.Kaplan (eds). Method in Enzymology, Academic press, New York 1984; 105: 121-126.

[17] Lowry OH, Rosenbrough NJ, Farr AL and Landall AJ. Protein estimation with Folin phenol reagent. J Biol Chem. 1951; 193:265.

[18] Marklund S and Marklund G. Involvement of the superoxide anion radical in autooxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974; 47: 469-474

[19] Roe JH, and Kuether CA. The Determination of Ascorbic Acid in Whole Blood and Urine through the 2,4-Dinitrophenylhydrazine Derivative of Dehydro ascorbic Acid. J Biol Chem. 1943; 147:399-407.

[20] Hanahan D, and Weinberg RA. The hallmarks of cancer. Cell. 2000; 100: 57–70.

[21] Boutwell RK. The function and mechanism of promoters of carcinogenesis. CRC Critical Reviews in Toxicology. 1974; 2: 419–443.

[22] Parmar J, Sharma P, Verma P, Sharma P, Goyal PK. Chemopreventive Action of Syzygium cumini on DMBA - induced Skin Papillomagenesis in Mice. Asian Pacific J Cancer Prev. 2009; 11: 261-265

[23] Copeland ES. A national institutes of health workshop report. Free radicals in promotion-A chemical pathology study section workshop. Cancer Res. 1983; 43: 5631–5637.

[24] Cerrutti PA. Peroxidant states and tumor promotion science (Washington DC). 1985; 227: 375–381.

[25] Kohen R and Nyska A, Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification, Toxicol Pathol. 2002; 30: 620–650.

[26] Gregus Z, Fekete, T, Halaszi E, Klaassen, CD. Lipoic acid impairs glycine conjugation of benzoic acid and renal excretion of benzoyl glycine. Drug Metabolism and Disposition 1996; 24: 682–688.

[27] Meister A. Glutathione, ascorbate and cellular protection. Cancer Res. 1994; 54s: 1969-1975.

[28] Jainu M, Devi CSS . Antioxidant effect of methanolic extract of Solanum nigrum berries on aspirin –induced gastric mucosa injury. Indian J Clin Biochem. 2004; 19: 57-61.

[29] Amin A. Chemopreventive effect of chlorella on the antioxidant system in DMBA-induced oxidative stress in liver. Int J Pharmacol. 2008; 4(3):169-176.

[30] Kumar A, IIavarasan R, Jayachandran T, Decaraman M, Aravindhan P, Padmanabhan N and Krishnan MRV. Phytochemicals investigation on a Tropical plant, Syzygium cumini from Kattuppalayam, Erode District, Tamil Nadu, South India. Pakistan Journal of Nutrition. 2009; 8(1): 83-85.

[31] Bhatia IS, Bhajaj KL. Chemical constituents of the seeds and bark of Syzygium cumini. Planta Medica. 1975; 28: 346–352.

[32] Sakagami H, Jiang Y, Kusama K, Atsumi T, Ueha T, Toguchi M, Iwakura I, Satoh K, Ito H, Hatano T and Yoshida T. Cytotoxic activity of hydrolysable tannins against human oral tumor cell lines – a possible mechanism. Phytomedicine. 2000; 7: 39–47.

[33] Yang LL, Lee CY and Yen KY. Induction of apoptosis by hydrolysable tannins from Eugenia jambos L. on human leukemia cells. Cancer Letters. 2000; 157: 65–67.

[34] Sen S, Makkar HPS and Becker K. Alfalfa saponins and their implication in animal nutrition. Journal of Agricultural and Food Chemistry. 1998; 46: 131–140.

[35] Ki Won Lee, Hyong Joo Lee, Young-Joon Surh, and Chang Yong Lee. Vitamin C and cancer chemoprevention: reappraisal1–3. Am J Clin Nutr 2003; 78: 1074–8.

Metadata

Repository Staff Only: item control page