Prevention of endotoxin-induced uveitis in rabbits by Triphala, an Ayurvedic formulation

Suresh Kumar Gupta, V. Kalaiselvan, Shyam Sunder Agrawal, Sushma Srivastava, Rohit Saxena, Int J Cur Biomed Phar Res. (2011) Prevention of endotoxin-induced uveitis in rabbits by Triphala, an Ayurvedic formulation. [Journal (Paginated)]

Full text available as:

PDF (Prevention of endotoxin-induced uveitis in rabbits by Triphala, an Ayurvedic formulation) - Published Version
Available under License Creative Commons Attribution No Derivatives.



Purpose: Triphala (TA) is an Ayurvedic formulation used to treat various disorders. The present study was designed to investigate the anti-inflammatory effect of TA aqueous extract on experimental uveitis in the rabbit. Methods: Anterior uveitis was induced in rabbits by intravitreal injection of lipopolysaccharide from Eschericha coli after pretreatment with TA aqueous extract. Subsequently the anti-inflammatory activity of TA was evaluated by grading the clinical signs and estimating the inflammatory cell count, protein, and TNF-α level in the aqueous humour. Results: The anterior segment inflammation in the control group was significantly higher than in TA and prednisolone treated groups, as observed by clinical grading. The inflammatory cell count in the control group was 31.23 ± 0.80 × 105cells/ml, whereas it was 3.29 ± 0.47 × 105cells/ml (P < 0.0001 vs. control) and 1.31 ± 0.31 × 105 (P < 0.0001 vs. control) cells/ml in the TA and prednisolone treated groups, respectively. The protein content of the aqueous humour was 15.43 ± 0.54, 3.13 ± 0.35 (P < 0.0001 vs. control), and 1.96 ± 0.39 (P < 0.0001 vs. control) mg/ml in the control, TA and prednisolone treated groups respectively. The aqueous TNF- α level in the control group was 942.20 ± 6.46 pg/ml and was 261.30 ± 13.60 (P < 0.001 vs. control) and 104.00 ± 4.50 (P < 0.0001 vs. control) pg/ml in the TA and prednisolone treated groups, respectively. Conclusions: Topical administration of aqueous extract of TA prevented uveitis in endotoxin-induced experimental rabbits.

Item Type:Journal (Paginated)
Keywords:Triphala, Uveitis, Endotoxin, TNF-α.
ID Code:7327
Deposited By:CurrentSciDirect Publications, International Journal of Current Biomedical and Pharmaceutical Research (IJCBPR)
Deposited On:02 May 2011 16:51
Last Modified:02 May 2011 16:51

References in Article

Select the SEEK icon to attempt to find the referenced article. If it does not appear to be in cogprints you will be forwarded to the paracite service. Poorly formated references will probably not work.

[1] Gritz DC, Wong IG. Incidence and prevalence of uveitis in Northern California; the Northern California Epidemiology of Uveitis Study. Ophthalmol.2004; 111: 491-500.

[2] Umesh CS, Yadav, Subramanyam S, Ramana KV. Prevention of endotoxin-induced uveitis in rats by benotiamine, a lipophilic analogue of vitamin B1. Invest Ophthalmol Vis Sci.2009; 50: 2276-2282.

[3] Gupta SK, Agarwal R, Srivastava S, Agrawal P, Agrawal SS, Saxena R, Galpalli N. The anti-inflammatory effects of Curcuma longa and Berberis aristata in endotoxin induced uveitis in rabbits. Invest Ophthalmol Vis Sci.2008; 49:4036-4040.

[4] Zhang H, Li X, Li X . Study on the effect and mechanism of qinggan xiehuo decoction in treatment of endotoxin-induced uveitis in rabbits. Zhongguo Zhong Xi Yi Jie He Za Zhi. 1196;16:95-98

[5] Rosenbaum JT, McDevitt HO, Guss RB, Egbert PR . Endotoxin-induced uveitis in rats as a model for human disease. Nature.1980; 286:611–613

[6] Li Q, Peng B, Whitcup SM, Jang SU, Chan CC. Endotoxin induced uveitis in the mouse: susceptibility and genetic control. Exp Eye Res. 1995; 61:629–632.

[7] Beutler B, Milsark IW, Cerami AC. Passive immunization against cachectin/tumor necrosis factor protects mice from lethal effect of endotoxin. Science. 1985; 229:869–871

[8] Lehmann V, Freudenberg MA, Galanos C. Lethal toxicity of lipopolysaccharide and tumor necrosis factor in normal and D-galactosamine-treated mice. J Exp Med.1987; 165:657–663.

[9] Mc lntyre A. Herbal treatment of children: western and Ayurvedic perspectives. Elsevier Health Sciences. 1985,

[10] Ayurvedic pharmacopoeia committee. The Ayurvedic Formulary of India, Part I, 2nd English ed. New Delhi: Controller of Publications 2003.

[11] Kroes BH, Van den Berg AJ, Quarles van Ufford HC, Van Dijk H, Labadie RP . Anti-inflammatory activity of gallic acid. Planta-Med. 1992; 58: 499-504.

[12] Kim SH, Jun CK, Suk K, Choi BJ, Lim H, Park S, Lee SH, Shin HY, Kim DK, Shin TY. Gallic Acid Inhibits Histamine Release and Pro-inflammatory Cytokine Production in Mast Cells. Toxicol Sci. 2006; 91:123-131.

[13] Reddy DB, Reddanna P. Chebulagic acid (CA) attenuates LPS-induced inflammation by suppressing NF-kappaB and MAPK activation in RAW 264.7 macrophages. Biochem Biophys Res Commun. 2009; 381: 112-117.

[14] Hamalainen M, Nieminen R, Vuorela P, Heinonen M, Moilanen E. Anti-inflammatory effects of flavonoids: genistein, kaempferol, quercetin, and daidzein inhibit STAT-1 and NF-kappaB activations, whereas flavone, isorhamnetin, naringenin, and pelargonidin inhibit only NF-kappaB activation along with their inhibitory effect on iNOS expression and NO production in activated macrophages. Mediators In Inflamm. 2007;45:673.

[15] Rasool M, Sabina EP. Anti inflammatory effect of the Indian Ayurvedic herbal formulation Triphala on adjuvant-induced arthritis in mice. Phytother Res.2007; 21:889-894.

[16] Abraham S, Kumar MS, Sehgal PK, Nitish S, Jayakumar ND. Evaluation of the inhibitory effect of triphala on PMN-type matrix metalloproteinase (MMP-9). J Periodontol. 2005; 76:497-502.

[17] Biradar YS, Jagatap S, Khandelwal KR, Singhania SS. Exploring of Antimicrobial Activity of Triphala Mashi—an Ayurvedic Formulation. Evid Based Complement Alternat Med. 2008; 5: 107–113.

[18] Ruiz-Moreno JM, Thillaye B, de Kozak Y. Retino-choroidal changes in endotoxin-induced uveitis in the rat. Ophthlmic Res.1992; 24:162-168.

[19] Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ . Protein measurement with the Folin-phenol reagent. J Biol Chem.1951; 193:265-275.

[20] Tuaillon N, Shen de F, Berger RB, Lu B, Rollins BJ, Chan CC . MCP-1expression of T lymphocyte subtype in endotoxin induced uveitis. Invest Ophthalmol Vis Sci.2002; 43:1493-1498.

[21] Avunduk AM, Avunduk MC, Oztekin E, Baltaci AK. Characterization of T lymphocyte subtypes in endotoxin-induced uveitis and effect of pentoxifylline treatment. Curr Eye Res.2002; 24:92-98.

[22] Reddy DB, Reddy TCM, Jyotsna G, Sharan S, Priya N, Lakshmipathy V, Reddanna P. Chebulagic acid, a COX–LOX dual inhibitor isolated from the fruits of Terminalia chebula Retz., induces apoptosis in COLO-205 cell line. J Ethnopharmacol.2009; 124: 506-512.


Repository Staff Only: item control page