Total Antioxidant Activity in Normal Pregnancy

Adiga, US and Adiga, MNS (2009) Total Antioxidant Activity in Normal Pregnancy. [Journal (On-line/Unpaginated)]

Full text available as:

PDF - Published Version
Available under License Creative Commons Attribution No Derivatives.



Objective: Pregnancy is a state, which is more prone for oxidative stress. Various studies report development of a strong defence mechanisms against free radical damage, as the pregnancy progresses. Aim of our study is to assess the antioxidant status by measuring the total antioxidant activity. Methods: Total antioxidant activity was assayed by Koracevic’ et al’s method, with the plasma of twenty five pregnant women (with normal blood pressure) as test group and twenty five age matched non-pregnant women as control group. All complicated pregnancies are excluded from the study. Results: Highly significant decline (P< 0.001) in antioxidant activity was observed in pregnant women with a value of 1.40 ± 0.25mmol/l, as compared to controls, 1.63 ± 0.21 mmol/l. Conclusion: Reduction in total antioxidant activity could be due to the fall in individual antioxidant levels. But several studies report an elevated enzymatic and non-enzymatic antioxidants during pregnancy. Any way total antioxidant activity is not a simple sum of individual antioxidants, but the dynamic equilibrium & cooperation between them. So inspite the rise in individual antioxidants , total antioxidant activity may be low. Further studies need to be done with antioxidant activity as a marker of complicated pregnancies like pregnancy induced hypertension.

Item Type:Journal (On-line/Unpaginated)
Keywords:Pregnancy, Antioxidant activity, Oxidative stress
Subjects:JOURNALS > Online Journal of Health and Allied Sciences
ID Code:6674
Deposited By:Kakkilaya Bevinje, Dr. Srinivas
Deposited On:14 Nov 2009 11:38
Last Modified:11 Mar 2011 08:57

References in Article

Select the SEEK icon to attempt to find the referenced article. If it does not appear to be in cogprints you will be forwarded to the paracite service. Poorly formated references will probably not work.

1. Toescu V, Nuttall SL, Martin U, Kendall MJ, Dunne F. Oxidative stress and normal pregancy. Clinical Endocrinology 2002;57(5):609-13.

2. Fridovich I. Hypoxia and oxygen toxicity. Adv Neurol 1979;26:255–9.

3. Dotsch J, Hogen N, Nyul Z, Hanze J, Knerr I, Kirschbaum M, Rascher W. Increase in endothelial nitric oxide synthase and endothelin–1 mRNA expression in human placenta during gestation. Eur J Obstet Gynecol Reprod Biol 2001;97:163–7.

4. Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. Method for the measurement of antioxidant activity in human fluids. J Clin Pathol 2001;54:356-61.

5. Wisdom SJ, Wilson R, McKillop JH, Walker JJ. Antioxidant systems in normal pregnancy and in pregnancy-induced hypertension. Am J Obstet Gynecol 1991;165:170–4.

6. Ilouno LE, Shu EN, Igbokwe GE. An improved technique for the assay of red blood cell superoxide dismutase (SOD) activity. Clin Chim Acta 1996;247:1–6.

7. Hubel CA, Kagan VE, Kisin E, McLaughlin MK, Roberts JM. Increased ascorbate radical formation and ascorbate depletion in plasma from women with preeclampsia: implications for oxidative stress. Free Radic Biol Med 1997;23:597–609.

8. Patil SB, Kodliwadmath MV, Sheela K. Lipid peroxidation and nonenzymatic antioxidants in normal pregnancy. J Obstet Gynecol India 2006;56(6):399-401.

9. Qanungo S, Mukherjea M. Ontogenic profile of some antioxidants and lipid peroxidation in human placental and fetal tissues. Mol Cell Biochem 2000;215:11–19.

10. Watson AL, Skepper JN, Jauniaux E, Burton G J. Changes in concentration, localization and activity of catalase within the human placenta during early gestation. Placenta 1998;19:27–34.

11. Watson AL, Palmer ME, Jauniaux E, Burton G J. Variations in expression of copper/zinc superoxide dismutase in villous trophoblast of the human placenta with gestational age. Placenta 1997;18:295–9.

12. Uotila J, Tuimala R, Aarnio T, Pyykko K, Ahotupa M. Lipid peroxidation products, selenium-dependent glutathione peroxidase and vitamin E in normal pregnancy. Eur J Obstet Gynecol Reprod Biol 1991;42:95–100.

13. Tamura T, Olin KL, Goldenberg RL, Johnson KE, DuBard MB, Keen CL. Plasma extracellular superoxide dysmutase activity in healthy pregnant women is not influenced by zinc supplementation. Biol Trace Elem Res 2001;80:107–14.

14. Kharb S. Total free radical trapping antioxidant potential in pre-eclampsia. Int J Gynaecol Obstet 2000;69:23–6.

15. Wang YP, Walsh SW, Guo JD, Zhang JY. Maternal levels of prostacyclin, thromboxane, vitamin E, and lipid peroxides throughout normal pregnancy. Am J Obstet Gynecol 1991;165:1690–4.

16. Yoshioka T, Ando M, Taniguchi K, Yamasaki F, Motoyama H. Lipoperoxidation and antioxidant substances in the human placenta during gestation. Nippon Sanka Fujinka Gakkai Zasshi 1990;42:1634–40.

17. Myatt L, Miodovnik M. Prediction of preeclampsia. Semin Perinatol 1999;23:45–57.

18. Roberts JM, Taylor RN, Musci TJ, Rodgers GM, Hubel CA, McLaughlin MK. Preeclampsia: an endothelial cell disorder. Am J Obstet Gynecol 1989;161:1200–04.

19. Poranen AK, Ekblad U, Uotila P, Ahotupa M. Lipid peroxidation and antioxidants in normal and pre-eclamptic pregnancies. Placenta 1996;17:401–05.

20. Watson AL, Palmer ME, Jauniaux E, Burton GJ. Variations in expression of copper/zinc superoxide dismutase in villous trophoblast of the human placenta with gestational age. Placenta 1997;18:295–9.

21. Poranen AK, Ekblad U, Uotila P, Ahotupa M. The effect of vitamin C and E on placental lipid peroxidation and antioxidative enzymes in perfused placenta. Acta Obstet Gynecol Scand 1998;77:372–6.

22. Wang Y, Walsh SW. Antioxidant activities and mRNA expression of superoxide dismutase, catalase, and glutathione peroxidase in normal and preeclamptic placentas. J Soc Gynecol Investig 1996;3:179–84.

23. Mohindra A, Kabi BC, Kaul N, Trivedi SS. Vitamin E and carotene status in preeclamptic pregnant women from India. Panminerva Med 2002;44:261-4.


Repository Staff Only: item control page