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Antibacterial Activity of Culture Extracts of Penicillium chrysogenum PCL501: Effects of Carbon Sources

Onyegeme-Okerenta, BM and Chinedu, SN and Okafor, UA and Okochi, VI (2009) Antibacterial Activity of Culture Extracts of Penicillium chrysogenum PCL501: Effects of Carbon Sources. [Journal (On-line/Unpaginated)]

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Abstract

Penicillium chrysogenum PCL501 produced β-lactam antibiotics when fermented with different agro-wastes: cassava shavings, corncob, sawdust and sugarcane pulp. In vitro antibacterial activity of the culture extracts was tested against four clinical bacterial isolates, namely, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa. All the culture extracts and standard drug (commercial Benzyl Penicillin) inhibited the growth B. subtilis and E. coli; the potency varied with carbon source. Antibacterial activity of extracts from cultures containing cassava shavings and sugarcane pulp was comparable with that of the standard drug. The MIC against the susceptible organisms was 0.20mg/ml for the standard drug and ranged from 0.40 to 1.50mg/ml for the culture extracts. Neither the culture extracts nor the standard drug inhibited K. pneumoniae and P. aeruginosa; the bacterial strains produced β-lactamase enzymes. Cassava shavings and sugarcane pulp are indicated as suitable cheap carbon sources for the production of antibiotics by Penicillium chrysogenum PCL501.

Item Type:Journal (On-line/Unpaginated)
Keywords:Agro-wastes, Penicillium chrysogenum PCL501, Antibiotic production, Phenyl penicillin, Antibacterial activity, β-lactamase
Subjects:JOURNALS > Online Journal of Health and Allied Sciences
ID Code:6567
Deposited By:Kakkilaya Bevinje, Dr. Srinivas
Deposited On:02 Jul 2009 02:45
Last Modified:11 Mar 2011 08:57

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