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Frequency Of Isolation Of Salmonella From Commercial Poultry Feeds And Their Anti-Microbial Resistance Profiles, Imo State, Nigeria

Okoli Ifeanyi, Charles and Ndujihe E, G and Ogbuewu P, I (2006) Frequency Of Isolation Of Salmonella From Commercial Poultry Feeds And Their Anti-Microbial Resistance Profiles, Imo State, Nigeria. [Journal (On-line/Unpaginated)]

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Abstract

This study was conducted to determine the frequency of isolation of salmonella and their microbial resistance profiles across different commercial poultry feeds sold in Imo State, Nigeria. Thirty-six bulk feed samples were colleted from 154 bag across different feed types and brands which included Guinea (GF), Top (TF), Vital (VF), Extra (EF), Animal care (AF) and livestock (LF) feeds. The salmonella isolated were tested against 14 anti-microbial drugs using the disc diffusion method. Bacterial load enumeration of the samples indicated a range of <30 colony forming unit (CFU) to overgrowth at 104 serial dilutions. Eight feed samples (22.2%) which cuts across the entire feed brands expect EF were positive for salmonella. The highest prevalence of 28.8% and 25.0% were recorded for LF and TF respectively, while VF, GF and AF had 11.1 and 10.0% respectively. Salmonella isolates showed high rates of resistance (51-100%) against nitrofurantoin, ampicillin, tetracycline and ceftriazole, while moderate rates (31-50%) were recorded for chloramphenicol, oxfloxacin and cotrimoxazole. Low resistance rates (1-30%) were on the other hand recorded against ciprofloxacin and amoxycillin clavulanate (Augumentine), whereas zero resistance was demonstrated against pefloxacin, gentamycin, streptomycin and nalidixic. Commercial feeds form important channels for the dissemination of multi-drug resistant salmonella in Imo State, Nigeria.

Item Type:Journal (On-line/Unpaginated)
Keywords:Salmonella, poultry feed, anti-microbial drugs, resistance profile, Nigeria
Subjects:JOURNALS > Online Journal of Health and Allied Sciences
ID Code:5167
Deposited By:Kakkilaya Bevinje, Dr. Srinivas
Deposited On:25 Sep 2006
Last Modified:11 Mar 2011 08:56

References in Article

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1. Ibe, SN. An introduction to genetics and animal breeding, Nigeria: Longman; 1998.

2. Saidu, L. Abdu, PA and Abdullahi, SU. Disease of turkeys diagnosed in Zaria from 1982-1991. Bulletin of Animal Health Production Africa 1994; 42:25-30.

3. Halle, PO, Umoh, JU and Abdu, PA. Diseases of poultry in Zaria, Nigeria: A ten year analysis of clinical records. Nigerian Journal of Animal Production 2002; 29: 1998; 25 (1): 88-92.

4. Bale OOJ, Sekoni, AA and Kwanashie, CN. A case study of possible health hazards associated with poultry houses. Nigerian Journal of Animal Production 2002; 29: 102-111.

5. Jones, PW. Salmonella in animal wastes and hazards for other animals and humans from handling animal wastes. In: Inter. Sym. on Salmonella and Salmonellosis. Ploufragan, France 1992; p. 280-284.

6. Seifert, HSH. Tropical animal health. Bisston/London: Kluwer Academic Publishers; 1996.

7. Henzler, DJ and Opitz, HM. The role of mice in the epizootiology of Salmonella enteritidis infections on chicken layer farmers. Avian Diseases 1992; 36:625-1631.

8. Hayashi, S and Yamazaki, H. Rapid and simple method of detecting salmonella in chicken feaces using polymyxin-cloth enzyme immunoassay. Food Microbiology 1996; 13:103-107.

9. Omede, AA. Quality assessment of commercial poultry feeds sold in Nigeria. B. Agric. Tech. Project reports, Federal University of Technology, Owerri, Nigeria. 2003.

10. Uwaezuoke, JC, Ogbbulie, JN, Njoku, AJ, et al. Antibiotics sensitivity patterns of bacterial isolates from poultry feed. International Journal Environmental Health Human Development 2000; 1(2): 23-28.

11. Wilson, JE. Raw materials-Animal proteins. Proceedings of the society of feed technologists. 1990.

12. Garland, PN. Salmonella prevention and salmonella inhibitors in poultry feed. Technical Publication of BOCM Paul Ltd, Ipswich, Suffolk, England. 1996; p. 28.

13. Okoli, IC, Herbert, U, Ozoh, PTE and Udedibie, ABI. Anti-microbial resistance profile of E. coli isolates from commercial poultry feeds and feed raw materials. Animal Research International 2005a; Accepted for publication.

14. Okoli, IC Studies on anti-microbial resistance among E. coli isolates from feeds and poultry production units, Ph. D. Thesis, Federal University of Technology, Owerri, Nigeria. 2004. p. 218.

15. Jacoby, GA and Archer, GL. New mechanism of bacterial resistance to anti-microbial agents. New England Journal of Medicine 1991; 324:601-912.

16. Okoli, IC, Okeudo, NJ and Nwosu, CI et al. Drug management of anti-microbial resistance in avian bacterial pathogen infections in Nigeria. International Journal Environmental Health and Human Development 2002; 3(1): 39 – 48.

17. Ogbulie, JN and Okpokwasili, GC. Efficacy of chemotherapeutic agents in controlling bacterial diseases of cultured fish. J. Aqua. Trop.1999; 13: 61-72.

18. Cheesbrough, M. Microbiological test. In: District laboratory practice in tropical countries. Part 2. Cambridge: Cambridge University Press; 2000.

19. Gillies, RR and Dodds, TC. Bacteriology illustrated, 4th Ed. Edinburgh and London: Churchill Livingstones; 1976.

20. Bauer, AW, Kirby, WMM, Sherris, JC and Turck, M. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology 1966; 36: 493 - 496.

21. NCCLS. Performance standard of anti-microbial disk and dilution susceptibility tests for bacteria isolated from animals. Approved standards, M 31 – A, 1999; 19 (11).

22. Jones, PW, Collins, P., Brown GTH and Aitken, M. Transmission of Salmonella mbandaka to cattle from contaminated feed. Journal of Hygiene 1982; 88:255-263.

23. Williams, JE. Salmonella in poultry feeds- a worldwide review. Part III. Methods in control and elimination. World’s Poultry Journal 1981; 37; 97-105.

24. Maciorowski, KG, Jones, TF, Pillai, SD and Ricke, SC. Incidence, source and control of food-borne Salmonella spp. in poultry feeds. World’s Poultry Journal 2004; 60(5): 446-457.

25. Ekwuagana, IC. Frequency of isolation of salmonella from feed raw materials and their anti-microbial resistance profile. B. Agric. Tech. Project Report, Federal University of Technology, Owerri, Nigeria. 2004.

26. Singer, JT, Opitz, HM, Gershman, M. et al. Molecular characterization of Salmonella enteritidis isolates from Maine poultry and poultry farm environment. Avian Diseases 1992; 36: 324-333.

27. Esonu, BO. Animal nutrition and feeding: A functional approach. Owerri, Nigeria: Ruzeal and Ruksons Associates Memory Press. 2000.

28. Chah, KF, Okafor, SC and Oboegbulem, SI. Anti-microbial resistance of none clinical E. coli strains from chicken in Nsukka, Southeastern Nigeria. Nigerian Journal of Animal Production 2003; 30 (1): 101-106.

29. Okoli, IC, Chah, KF, Herbert, U et al. Anti-microbial resistance of non-clinical E. coli isolates from a commercial layer poultry farm in Imo state, Nigeria. International Journal of Natural and Applied Sciences 2005b; 1(1): 68-77.

30. Okoli, IC, Chah, KF, Ozoh, PTE and Udedibie, ABI. Anti-microbial resistance profile of E. coli isolates from tropical free-range chickens. Online Journal of Health and Allied Sciences 2005c; 3(3): http://www.ojhas.org/issue15/2005-3-3.htm 12/19/2005.

31. Anyanwu, BB. Causes of embryo mortalities in breeder turkey eggs in Owerri, Nigeria. Unpublished field data. 2001.

32. Okoli, IC. Salmonella strains Isolated from a turkey farm in Owerri, Nigeria. Unpublished field data. 2003.

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