Cogprints

Biological Pacemakers

G, Rajesh and Francis, Johnson (2005) Biological Pacemakers. [Journal (Paginated)]

Full text available as:

[img] HTML
31Kb
[img]
Preview
 PDF
132Kb

Abstract

Genetically engineered pacemakers could be a possible alternative to implantable electronic devices for the treatment of bradyarrhythmias. The strategies include upregulation of beta adrenergic receptors, conversion of myocytes into pacemaker cells and stem cell therapy. Pacemaker activity in adult ventricular myocytes is normally repressed by the inward rectifier potassium current (IK1). The IK1 current is encoded by the Kir2 gene family. Use of a negative construct that suppresses current when expressed with wild-type Kir2.1 is an experimental approach for genesis of genetic pacemaker. hyperpolarisation activated cyclic nucleotide gated (HCN) channels which generate If current, the pacemaker current of heart can be delivered to heart by using stem cell therapy approach and viral vectors. The unresolved issues include longevity and stability of pacemaker genes, limitations involved in adenoviral and stem cell therapy and creation of genetic pacemakers which can compete with the electronic units.

Item Type:Journal (Paginated)
Keywords:Gene therapy, Pacemaker current, HCN channels
Subjects:JOURNALS > Indian Pacing and Electrophysiology Journal
ID Code:4599
Deposited By: Indian Pacing and Electrophysiology, Journal
Deposited On:06 Jan 2006
Last Modified:11 Mar 2011 08:56

References in Article

Select the SEEK icon to attempt to find the referenced article. If it does not appear to be in cogprints you will be forwarded to the paracite service. Poorly formated references will probably not work.

1. Edelberg JM, Aird WC, Rosenberg RD. Enhancement of murine cardiac chronotropy by molecular transfer of the human b2 adrenergic receptor cDNA. J Clin Invest. 1998;101:337-343.

2. Miake J, Marban E, Nuss HB. Gene therapy: biological pacemaker created by gene transfer. Nature. 2002;419:132-133.

3. Potapova I, Plotnikov A, Lu Z. Human mesenchymal stem cells as a gene delivery system to crate cardiac pacemakers. Circulation research. 2004;952-959.

4. Zhou YY,Wang SQ, Zhu WZ,Chruscinski A, Kobilka BK, Ziman B,Wang S,Lakatta EG, Cheng H, Xiao PP. Culture and adenoviral infection of adult mouse cardiac myocytes;methods for cellular genetic physiology. Am J Physiol (Heart Circ physiol). 2000;279:H429-H436.

5. Hamm A, Krott N, Breibach I, Blindt R, Bosserfoff AK. Efficient transfection method for primary cells. Tissue ENG. 2002;8:235-245.

6. Di Francesco D. Pacemaker mechanism in cardiac tissue. Annu Rev Physiol. 1993; 55:455-472.

7. Ludwig A, Zong X,Jeglitsch M. A family of hyperpolarisation activated mammalian cation channels. Nature 1998; 393:587-591.

8. Andreas Ludig et al. HCN channels, From genes to function. In: Douglas p . Zipes, Jose Jalife, ed: Cardiac electrophysiology, From cell to bedside. Fourth edition. Saunders. pp. 59-65.

9. Moosmang S, Stieber J, Zong X, Biel M, Hofmann F, Ludwig A. Cellular expression and functional characterization of four hyperpolarization-activated pacemaker channels in cardiac and neuronal tissues. Eur J Biochem. 2001;268:1646-52

10. Biel M, Schneider A, Wahl C. Cardiac HCN channels: structure, function, and modulation. Trends Cardiovasc Med. 2002;12:206-12.

11. Lopatin AN, Nichols CG. Inward rectifiers in the heart: an update on I(K1). J Mol Cell Cardiol. 2001 ;33:625-38.

12. Nichols CG, Makhina EN, Pearson WL, Sha Q, Lopatin A. Inward rectification and implications for cardiac excitability. Circ Res. 1996 ;78(1):1-7.

13. Qu J, Plotnikov AN, Danilo P Jr, Shlapakova I, Cohen IS, Robinson RB, Rosen MR. Expression and function of a biological pacemaker in canine heart. Circulation. 2003; 107(8):1106-9.

14. Plotnikov AN, Sosunov EA, Qu J, Shlapakova IN, Anyukhovsky EP, Liu L, Janse MJ, Brink PR, Cohen IS, Robinson RB, Danilo P Jr, Rosen MR. Biological pacemaker implanted in canine left bundle branch provides ventricular escape rhythms that have physiologically acceptable rates. Circulation. 2004;109 (4):506-12.

15. Valiunas V, Weingart R, Brink PR. Formation of heterotypic gap junction channels by connexins 40 and 43. Circ Res. 2000; 86(2):E42-9.

Metadata

Repository Staff Only: item control page

Cogprints is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.